Native™ Antibody Discovery by B cell Sorting https://www.creative-biolabs.com/native-antibody/antigen-specific-b-cell-isolation.htm Monoclonal antibody (mAb) has experienced the iterative update from mouse mAb, chimeric antibody, and humanized antibody to whole human antibody since the emergence of hybridoma technology in 1975. The overall trend is to increase the humanization of antibodies due to the rapid human anti-mouse immune reaction in the human body. This species’ immune reaction not only accelerates the clearance of murine antibodies leading to a decreased titer but also causes adverse allergic reactions. At present, mature antibody production technologies, including hybridoma technology, phage display technology, and transgenic mouse technology, show different limitations, such as inevitable immunogenicity, low affinity, cost, and time consumption, the most important of which is that they cannot provide antibodies with naturally paired heavy chain and light chain.